Royal Society of Chemistry Advanced Mass Spectrometry-based Analytical Separation Techniques

The efficient analysis of polar and charged metabolites in biological samples remains a huge challenge in the field of metabolomics. Over the past years, novel mass spectrometrybased analytical tools have been developed to enable the sensitive and efficient profiling of polar ionogenic metabolites in various biological samples. This book gives the reader a comprehensive overview of these recent technological developments. Topics covered include the use of chemical labelling strategies for allowing the analysis of polar metabolites using reversedphase liquid chromatographymass spectrometry (RPLCMS) and the latest methodological developments in RPLCMS, hydrophilic interaction liquid chromatography (HILIC)MS and ionpair LCMS approaches. Attention is also paid to developments in nanoLCMS and capillary electrophoresismass spectrometry methods specifically for profiling polar metabolites in small volume biological samples. The utility of ionmobility MS and NMR spectroscopy will also be outlined. Sample preparation is the key part in the analytical workflow employed for metabolomics. Therefore, ample emphasis will be given on recent solidphase extraction and solidphase microextraction methods. Finally, analytical techniques for chiral metabolic profiling will also be considered. Discussing the stateoftheart of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field. This book has relevance... | Royal Society of Chemistry Advanced Mass Spectrometry-based Analytical Separation Techniques