Multi-Dimensional Liquid Chromatography : Principles, Practice, and Applications

Two-dimensional liquid chromatography (2D-LC) is finding increasingly wide application principally due to the analysis of mixtures of moderate to high complexity.Many industries are developing increasingly complex products that are challenging the separation capabilities of state-of-the-art 1D-LC and need new analytical methodologies with substantially more resolving power, and 2D-LC meets that need.This text, organized by two leaders in the field, establishes a sound fundamental basis for the principles of the technique, followed by a discussion of important practical considerations.The book begins with an introduction to multi-dimensional separations and a discussion of the history and development of the technique over the past 40 years, followed by several chapters that provide a theoretical basis for development of 2D-LC methods, including foundational concepts regarding separation complementarity, under-sampling, and dynamics of liquid chromatography separations.Instrumentation for 2D-LC is discussed extensively, including practical aspects such as interface selection and setup.Building on this foundation, two separate chapters are focused on method development for non-comprehensive and comprehensive separations, followed by a chapter dedicated to data analysis.Finally, applications of 2D-LC in several fields ranging from pharmaceutical analysis to polymer science are summarized. The book is an important resource for both students and practitioners who are already using 2D-LC or are interested in getting started in the field. Key Features:Demonstrates the conditions under which a 2D-LC method should be considered as an alternative to a 1D-LC methodEstablishes a sound fundamental basis of the principles of the technique, followed by guidelines for method optimizationProvides a single source for technical knowledge advances and practical guidance described in recent literatureAssists with the initial decision to develop a 2D-LC methodGuides the reader in developing a high-quality method that meets the needs of their application